The reactivity could be weakened by pretreatment with avidin and biotin. |
The endogeneous biotin was blocked by adding an excess amount of avidin followed by washing and addition of free biotin. |
Avidin strongly binds to biotin in raw eggs and in doing so blocks the absorption of this B vitamin, but the bond is released when avidin is denatured through heating. |
In the second step, an aliquot of the incubation mixtures containing C1-lnhibitor Reactant is added to microtiter wells precoated with avidin. |
The intestinal absorption of biotin, a vitamin included in yeasts, is actually inhibited by an antibiotin substance present in raw egg whites, avidin. |
This is because egg white contains a specific protein, avidin, that combines with biotin and thus prevents its absorption. |