| However, understanding of the DNA delivery process is still limited and the low efficiency of lipid-based transfection is a concern. |
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| Then, 4-6 hr after transfection, the cells were washed, fed with new medium, and allowed to grow for 36 hr. |
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| So, I celebrated by teaching her how to do a transfection, and then we went to lunch and talked about the goods and bads of science, very openly. |
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| Future studies should reveal other types of well-defined structure-function correlations for transfection in vivo in the presence of serum. |
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| Transient transfection was carried out using lipofectamine according to the protocol suggested by the manufacturer. |
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| Each transfection was performed in triplicate and experiments were repeated twice. |
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| Twenty-four hours after transfection, the cells were harvested and the plasmid DNA recovered. |
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| Two days after transfection had been initiated, cells were passaged onto coverslips. |
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| Twenty-four hours after transfection, cells were stimulated with ionomycin, phorbol 12-myristate 13-acetate, and calcium chloride. |
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| The incorporation of plasmids or prophages into the bacterial chromosomes is called transfection. |
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| More than a decade of research into nonviral gene delivery has yielded a number of synthetic cationic lipids and polymers with transfection activity. |
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| The global transfection reagent market is segmented on the basis of product, method, application, and region. |
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| After 10 min of incubation, transfection mixture was added to cell culture dishes. |
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| At different time points after LNA-anti-miR92a transfection, miR-92a quantitation and cell viability were assessed by qRT-real-time-polymerase chain reaction and MTT assays. |
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| Establishment of permanent human endothelial cells achieved by transfection with SV40 large T antigen that retain typical phenotypical and functional characteristics. |
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| To examine the effects of sodium butyrate on virus production, cells were exposed to sodium butyrate at various concentrations and times starting 16 h after transfection. |
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| Both transfection of human prochymase cDNA and injections of purified chymase into implanted sponges demonstrated that chymase was a powerful angiogenic factor. |
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| Transfection is a process whereby nucleic acid, primarily as recombinant DNA of known sequence and functionality, is placed in a target cell. |
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| Altogen Biosystems has developed a new generation of highly efficient Nanoparticle-based In Vivo Transfection Kit that it exclusively manufactures. |
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