Double-stranded oligos were multimerized using T4 DNA ligase in the presence of XhoI and SalI restriction enzymes to generate tandem arrays. |
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All the annealed double stranded oligos were cloned into the StuI site using blunt end ligation. |
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All oligos were obtained from Sigma Genosys and further purified using polyacrylamide gel electrophoresis. |
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Further experiments with small duplex oligos are planned to probe this issue. |
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For ACO genes, four degenerate oligos were synthesized on the sequences of three different conserved peptides. |
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These oligos were used with an appropriate upstream oligo to amplify fragments that were subcloned into pSM100 as described above. |
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Two single-stranded oligos, GAGAEXHO and GAGAESAL, were annealed to generate a double-stranded oligo with XhoI and SalI cohesive ends. |
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The technique utilizes a thermostable DNA ligase to ligate together perfectly adjacent oligos. |
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The sequence of the two nonconserved regions, NC1 and NC2, is shown, with the nucleotide substitutions in mutated oligos shown above the wild-type sequence. |
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These oligos are expected to allow researchers to achieve better results from multiplexing experiments. |
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New equipment includes 4 AKTA OligoPilots each capable of producing oligos from 500 umole to 50g scale of oligo per run. |
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Oligo arrays have improved dramatically in the past few years and we are delighted there are now oligos that meet our high standards. |
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These oligos are likely to permit researchers to attain better results from multiplexing experiments. |
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The astrocyte becomes important in later MS lesions, when it multiplies and eventually forms scar tissue that fills in where the oligos and myelin have been destroyed. |
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