Samples were allowed to equilibrate at this temperature for at least 10 min before measurements. |
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Functional residual capacity was measured by helium dilution over 7 min of rebreathing in closed circuit. |
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The root tips were macerated in a mixture of ethanol and hydrochloric acid for 2 min at room temperature. |
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After 10 min of incubation at room temperature, 1.25 ml of solubilized extracts were aliquoted into 1.7-ml microcentrifuge tubes. |
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From February 10th, televiewers will get to know the ten candidates throughout short 1 min 15 sec reports. |
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Pour the batter into the pan and bake for approximately 55 min or until the top of the cake is nicely brown and a cake tester comes out clean. |
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All samples were heated for 5 min in a water bath at the required temperature. |
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After 1 min of extraction, cells were sedimented and the supernatant was decanted. |
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Pollen was sedimented by centrifugation at 100 g for 2 min and the excess sucrose drawn off by pipette. |
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In each case, six fractions of light were given in 2 min intervals interlaced with 2 min intervals when the light was off. |
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An air barograph recorded atmospheric pressures every 1.5 min at a fixed station during the gravity survey. |
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During the interview after doing a brief 10 min test the psych said I would have difficulty in dealing with the course. |
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Follow processing time instructions and then apply the lightener to the roots 15-20 min before time is up. |
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The gel was stained with ethidium bromide for 30 min and subsequently destained for 3 hr. |
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Birds awoke within 3 min and were placed into small recovery cages before being released into their housing aviaries. |
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Wait 20-30 min to allow the stannous pyrophosphate to circulate throughout the body. |
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Approximately 20-30 min can be spent effleuraging the posterior side of the body and 10-15 min effleuraging the front of the legs and the arms. |
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The blood collected for HIV serology was allowed to clot for 30 min at room temperature. |
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The first two tiebreak sets occupied a total of 2hr 25 min as the opening round match inched along under floodlights. |
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The intact nuclei were incubated for 15 min after which they were gently pierced with two fire-polished glass micropipettes. |
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Leaves were kept in darkness for 30 min before recording the fluorescence parameters. |
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The beads were kept in suspension for 30 min by shaking at 400 rpm on a microfuge tube shaker at room temperature. |
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The slides were incubated for 30 min in a humidified chamber at room temperature. |
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Observations were spaced at least 10 min apart and consisted of a count of the number of tadpoles active and resting inactively. |
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The tissue was sandwiched between a positively charged glass slide and a siliconized coverslip and incubated for 30 min at room temperature. |
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Hearts were perfused for 5-7 min with nominally Ca free Tyrode's solution, then perfusion proceeded with added collagenase and albumin. |
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Hearts were excised quickly, placed on a Langendorff apparatus and perfused for 5 min with nominally Ca-free Tyrode's solution. |
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The mixture was pelleted by centrifugation at 13,000g for 8 min and the ethanol removed. |
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Material was spun at 6000 rpm for 1 min to pellet nucleic acid, and supernatant was removed to a clean tube. |
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The homogenate was incubated at room temperature for 5 min with agitation and then supplemented with 6 ml of chloroform. |
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Twenty slugs were filmed while receiving 10 swim-eliciting tail stimuli at a 2 min inter-trial interval. |
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It is not easily commutable to London, which is 1hr 50 min from the railway station. |
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Phosphorothioate modification was obtained using tetraethylthiuram disulfide for 15 min at room temperature as recommended by Applied Biosystems. |
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Dervaig is around 1hr 20 min drive north-westwards from Craignure, Mull's port for car ferry arrivals from Oban. |
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Add filets flesh-side down and marinate, covered for 30 min in refrigerator. |
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The gels were neutralized in 2 M TRIS for 5 min and subjected to fluorography by soaking for 30 min in Amplify. |
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Glass coverslips were washed with ethanol followed by distilled H2O and allowed to air dry for 30 min before sample adsorption. |
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Heat the oil in a frying pan, and cook the salmon skinside down for 5 min until crisp. |
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Excess solution was removed and fresh degassed buffer solution was added to the sample and allowed to incubate for 30-60 min before imaging. |
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The pots were carefully sprayed with pollutant solutions for 2 min and maintained in propagators in the growth cabinet as described above. |
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Leaves were then infiltrated with deionized water under vacuum three times for 4 min to ensure complete infiltration. |
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Surely my 30 min power nap during the day shouldn't have given me that effect? |
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The filtered solution was deaerated with dry argon for 10 min and the mold was sealed. |
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And he got off to a flying start in this year's series when he finished second in 12 min 29 sec in Monday's opening 2.2 mile race. |
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The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected. |
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Recent studies found that acidic solutions could increase the flow throughout the stems after 40 min of flushing. |
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Male behavior was recorded for 30 min by using a VHS video recorder on day 2 after spawning. |
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I was reading my book for the first 45 min or so, and when I looked up I felt terribly queasy. |
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Following additional washes with PBT, the slides were treated for 10 min with acetic anhydride and triethanolamine-HCl to reduce background. |
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Soluble and insoluble fractions were separated by centrifugation at 8 000 g for 30 min at the extraction temperature. |
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The eggs were exposed to ether vapor for 10 min and left in the fume cupboard for 30 min. |
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Spores were pretreated with BFA for 60 min and settled onto polystyrene for 5 min. |
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Pollen was germinated in vitro in liquid medium for 10 min and harvested by centrifugation. |
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It's action sequences, basically 2 hrs of its 2 hr. 45 min running time, are bravura for sure. |
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A hive entrance was blocked for 1 min, and four returning foragers were collected in individual plastic vials. |
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Swap the whisk for a wooden spoon as it thickens, and continue stirring occasionally for 5 to 10 min over medium heat. |
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In Missouri, sparrows and cowbirds arrived at nests at 13.5 and 14.7 min before sunrise, respectively. |
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The tubes were centrifuged at 5000 rpm for 2 min, and the supernatant discarded. |
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Add the tomatoes, chilli, turmeric, cayenne, ground coriander and salt, and cook for 3 min, stirring well. |
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To stabilize gas measurements, patients were asked to remain still on the ergometer for 3 min before exercising. |
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Add the garlic, parsley, thyme, salt and pepper and cook for 5 min or until the juices almost cook away. |
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Cook the potatoes in simmering salted water for 20 min or until tender. |
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Trials ended after 3 min of foraging or 1 min after all the birds flew back to their perches, after which the remaining seeds and husks were removed. |
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Two were gold medals, with one in the 5000m in 15 min 20 sec and the 10,000m in 32 min 44 sec, a silver in the steeplechase in 9.37 and a bronze medal in the half marathon. |
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Both vesicles and bilayer patches were found not to move laterally over the time range of 10 min and more, except at relatively high imaging forces. |
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Local media reported that suspect min Yingjun was a local villager suffering from a mental disorder. |
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Prior to feeding, colostrum containers were submersed in warm water for approximately 30 min for complete thawing, and their quality was estimated with a colostrometer. |
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Fuller was making overtures about hiring min to take over Star, and Wenner was desperate not to lose min as well. |
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Finally a dedicated software, interfaceable with record and verify systems used in the centers, was developed to obtain in vivo dosimetry results in less than 2 min after beam delivery |
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The top chamber was removed in a fume hood and allowed to vent for 1 min to remove any CO 2 present, the plants were then excised at the sand surface. |
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Samples are mixed gently for 10 s, then loaded into observation chambers and allowed to equilibrate for 60 min at room temperature before particle movements are recorded. |
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If we accept 2hr 10 min as the benchmark for a male marathoner, it is worthwhile considering that in 1987, just two runners raced inside that time. |
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The shoots were removed at the stem base and partially dried for 2-4 min in a microwave oven at 750 W to minimize the repartitioning of nutrients. |
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In experiments that test the effect of actin, cells were incubated for 30 min before experiments in medium containing 20 M cytochalasin D to disrupt actin filaments. |
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Before starting the experiments, oocytes were equilibrated for at least 20 min in substrate-free bathing medium to obtain a stable volume measurement. |
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Aliquots of 2 mL of the virus-photoproducts solution were placed in quartz phototubes, capped, and bubbled with oxygen for 5 min before being irradiated. |
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Spectra were measured at different temperatures, and samples were allowed to equilibrate at each temperature for at least 10 min before data collection. |
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The questionnaire, in Spanish, took approximately 45 min to administer. |
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This result indicates that negative consummatory contrast was produced when the subjects were allowed access to food pellets during the final 3 min of the session. |
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All patients were premedicated with pentazocine 30 min before the test. |
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After a further 15 min of perfusion with isosmolar perfusate, we added back leukocytes to the perfusate before instilling either acid or saline in the airway. |
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After incubation for half an hour, the sections were rinsed with tris buffer, peroxidase solution was added, incubated for 30 min and later rinsed with the buffer. |
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In the accompanying figure, the low tide lags or leads by 1 hr 2 min from its neighboring lines. |
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Shaking for 16 min resulted in weak plants with clorosis and epinasty symptoms in leaves. |
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After centrifugation at 12,000 rpm for 10 min with a microhematocrit centrifuge, hematocrit was immediately measured in a capillary tube. |
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The conical flask was shaken for 30 min and then kept in undisturbed condition for 30 min. |
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Total cell lysates were centrifuged at 2,000 x g for 10 min and the supernatants were used as cytosolic fractions. |
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Respiratory apnea followed the hyperpnea and lasted approximately 2 min when the animal was returned to the respirator. |
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Typically, 20-30 min of instruction was followed by approximately 20-30 min of independent seatwork on the material presented by the teacher. |
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The slides were then washed and destained for 5 min in distilled water and examined immediately under fluorescence microscope. |
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Asiatic acid, scopolamine and baclofen were administered 30 min before the test. |
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On day 1, 6 individuals were intravenously injected with normal saline solution at 8 AM, and then administered anthocyanin capsules 30 min later. |
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For untreated wastewater, 1 L was centrifuged for 15 min at 9,000 x g and concentrated with polyethylene glycol. |
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The tube was vortexed for 2-3 min to dislodge conidia from the insect and the concentration of conidia was determined using a haemocytometer. |
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Cut a 250g pack Cypriot halloumi into 6 slices, then chargrill or grill them until golden brown, about 1 min per side. |
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Briefly, the calves were restrained and sedated 20 min prior to the procedure and placed in left-lateral recumbency. |
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Twenty microliter blood was drawn from the postorbital artery in the 2nd and 10th min after injection, respectively. |
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Derwent's Ollie Pirt triumphed in the junior section in 1 min 30 sec, with club mates Thomas Nicholls and Emily Toman third. |
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Electrophoresis was performed for 90 min at 120 V. The gel was vacuum dried and then exposed to a phosphoscreen for 45 min. |
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In this study, we examine skin SIgA, staphylococci, HBD-2, and moisture content of the stratum corneum after 60 min and 120 min of exercise. |
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A typical chromatogram obtained for cTnI in a sample obtained 120 min after PTCA is shown in Fig. |
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Paradigmatic Problems that handles several famous combinatorial optimization problems as max cut, min coloring, optimal satisfiability tsp, etc. |
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After reporting to the laboratory at a standardized time subjects rested in the supine position for 10 min prior to blood collection. |
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Electrophoresis was run at 125 V constant voltage for 180 min and the run stopped after the bromophenol blue dye reached the end of the plate. |
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On the evening of the sixth day of hospitalization, 2 enemas were administrated 30 min apart to relieve prolonged constipation. |
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One unit was defined as the amount of protease that released one micromole of p-nitrophenolate per min in the assay conditions. |
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Cell plates were shaken for 5 min and the absorbance of each well was recorded on a DTX 800 Multimode Detector at 490 nm. |
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After 10 min of incubation, transfection mixture was added to cell culture dishes. |
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After 5 min of centrifugation, the super natant was recovered and the cTnT concentration measured without dilution. |
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All sessions were 10 min long and were conducted during math seatwork because that situation was consistently associated with problem behavior. |
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Samples unexposed to metals were stained with methylamine tungstate for 1 min so that the cells can easily be observed under microscope. |
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After incorporating all the pigment, the premix was blended for 10 min to deflocculate the pigments. |
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At 45 min postmortem, muscle samples for the analysis of glycogen and lactate contents were taken from the longissimus lumborum muscle at the 9th and 10th thoracic vertebrae. |
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The ocular blood flow velocity, external ear temperature and respiratory variables were obtained every 3 min during the resting, exercise, and recovery periods. |
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At the start of the study, a fetal cardiograph was recorded using the electronic device for monitoring fetal heart rate for 20 min in both groups. |
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Edema was measured plethysmographically 30, 60, 120 and 240 min later. |
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In a three-necked flask, 120 mmol of paraformaldehyde and 60 mmol of 6-amino-1-hexanol, and 100 mL of dioxane were mixed for about 20 min at room temperature. |
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The perchloric acid was neutralized by adding 2 M potassium bicarbonate, was shaken gently for 1 min, and was stored for a minimum of 5 min in a refrigerator. |
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For any given sample, an additional 3-6 min of mixing was performed if unhomogenized tissue remained after the initial 6 min homogenization period. |
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The replenishers reservoir should be at or above the MIN LEVEL mark. |
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