The results strongly suggest that some mRNA species are imported into sieve elements, which are enucleate, from neighbouring companion cells. |
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The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed. |
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Codon bias allows mRNA transcripts to be translated more quickly and accurately through efficient use of the transfer RNA pool. |
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A cDNA library of mRNA isolated from asparagus spears 24 h post-harvest was screened for peroxidases using homologous and heterologous probes. |
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Motors, such as RNA polymerases and ribosomes, produce trailing strands of mRNA and protein, respectively. |
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First, it is a highly conserved component of eukaryotic mRNA decay machinery. |
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Special proteins remove the noncoding regions from the mRNA before it is translated into protein at the ribosome. |
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Petals were detached from three flowers, pooled and used for mRNA isolation. |
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Processed pseudogenes may originate by reverse transcription from mature mRNA and subsequent insertion in the genome. |
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The levels of rbcS mRNA decreased to a minimum at 76 DAS, then increased to a maximum at 110 DAS, and finally declined. |
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Neuroglobin mRNA was also present in adrenal cells and the cells of the pancreatic islets of Langerhans. |
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The ligated mRNA is reverse transcribed using a general or gene-specific primer. |
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Intronic SSRs can affect gene transcription, mRNA splicing, or export to cytoplasm. |
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This rate closely matches the rate at which ribosomes translate along an mRNA sequence. |
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The double helix unwinds and the nucleotides follow basically the same base pairing rules to form the correct sequence in the mRNA. |
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In the third step, an RNA oligo that carries two primer sites is ligated to the decapped mRNA by T4 RNA ligase. |
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The present study is concerned with the control of expression through mRNA degradation. |
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This value was lower than the average amount of mRNA detected in all polysomes in leaves of arabidopsis and rapidly dividing yeast cells. |
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The building block cost for mRNA includes the nucleotides for an untranslated region and a polyA tail with empirically observed lengths. |
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Some operons are under attenuator control, in which transcription is initiated but is halted before the mRNA is transcribed. |
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Because each EST is identified as relating to an mRNA, it represents part of a gene that encodes proteins. |
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Clinical approaches use mRNA either for direct administration or for engineering of adoptively transferred dendritic cells. |
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After genetic information from DNA is transferred to messenger RNA, the mRNA data is transferred to the protein during what is called translation. |
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The parent strain S92 and ML-01 were grown in synthetic must, the total mRNA was extracted and analyzed in quadruplicate. |
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It is based on directly determining the expression of oncogenic risk factors by detecting the mRNA of the E6 and E7 proteins. |
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The TPA mRNA are used to made a copy of the cDNA, and it's then inserted into an expression vector and transfected into E. coli. |
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High protein and mRNA expression was also observed in the preoptic area, anterior hypothalamus, and habenular nucleus. |
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By in situ hybridization, MnSOD mRNA can be detected in arterioles, septal tips of alveolar ducts, endothelial cells, and in pleural mesothelium of hyperoxia-exposed rats. |
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When the PROGENSA PCA3 Assay is performed in the laboratory, the target mRNA molecules are isolated from the urine specimens by target capture. |
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To characterize this photic entrainment we examined frq expression in constant light, under which condition the mRNA and protein of this clock gene were strongly induced. |
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The mRNA containing the amber codon then leaves the nucleus and travels to the ribosome where it serves as a template for translation of a specific protein. |
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In wild-type and azygous fruit, TBG6 mRNA levels were high in the early stages of development, but then dropped dramatically after the breaker stage. |
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Some bind to mRNA and render it inactive, a process known as RNA interference. |
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Regional differences in expression of VEGF mRNA in rat gastrocnemius following 1 hr exercise or electrical stimulation. |
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Commonly, there are stimulatory signals in mRNA distinct from the shift site that greatly increase the level of frameshifting at the shift site. |
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Furthermore, significantly higher levels of loricrin and transglutaminase-3 mRNA were observed in the SM group. |
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The AUG mRNA codon codes for methionine and also acts as the start codon for all proteins. |
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Evidence against either a premature stop codon or the absence of Ob gene mRNA in human obesity. |
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Femoral mRNA levels of selected bone matrix proteins were determined by northern blot analysis. |
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Their dystrophin gene has a point mutation in the splice receptor site of intron 6 which leads to the deletion of exon 7 in the mRNA, to a frame shift, and a premature stop codon. |
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Thus, any mRNA codon that happens to be on the ribosome at any one time will solicit the binding only of the tRNA with the appropriate anticodon, which will align the correct amino acid for addition to the chain. |
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This causes a frame shift in the mRNA and a premature stop codon leading to a truncated and non-functional dystrophin protein which is subsequently degraded in the muscle cells. |
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Pre-mRNA is extensisextensivelythrough splicing beditedthroughure mRNA isplicinged and ready for translation before ribosome, the cellular organelle that serves as the site of protein synthesis. |
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Marker polymorphisms were used to determine the allelic origin of lactase mRNA transcripts. |
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Differential regulation of polysome mRNA levels in mouse Hepa-1c1c7 cells exposed to dioxin. |
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Fourth, mutations within introns that affect the specific nucleotide sequences that direct intron splicing may result in an mRNA that still contains an intron. |
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Increscent of mRNA concentration with increscent requirement protein cause property response to the hormones. |
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Jukes pointed out that the code constraints on the mRNA sequence required for this translation mechanism is still preserved. |
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With the mitochondrial RNA processing, individual mRNA, rRNA, and tRNA sequences are released from the primary transcript. |
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Hoxb6 mRNA is detected in neural tube and somites, with an anterior boundary in somites that contribute to the sixth prevertebra. |
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The act of translation requires the stepwise decoding of the mRNA one codon at a time. |
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Anemonin is a natural bioactive compound that can regulate tyrosinase-related proteins and mRNA in human melanocytes. |
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Detection of osteoprotegerin and TNF-alpha mRNA in ankylotic stapes footplates in connection with measles virus positivity. |
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Hepatic GH binding was determined by radioreceptor assay and hepatic IGF-I mRNA levels were determined by quantitative slot-blot analysis. |
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The coding RNA section deals with mRNA metabolism and the flow of genetic information from the DNA storage site to the ribosomes. |
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Downregulation of kappa opioid receptor mRNA levels by chronic ethanol and repetitive cocaine in rat ventral tegmentum and nucleus accumbens. |
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Transcription of mRNA and transfer of the amino acids by specific tRNA are vital steps in cellular protein synthesis on the surface of ribosomes. |
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We focused on the PAF1 complex which has been previously implicated in gene transcription, cell cycle control and mRNA surveillance. |
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Chromosomal translocation t results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor. |
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The team also created a system that would cause yeast cells to edit the mRNA, replacing the uridine in the codon that signals a halt in protein production with pseudouridine. |
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Mitogen activated protein kinase p38 controls the expression and posttranslational modification of tristetraprolin, a regulator of tumor necrosis factor alpha mRNA stability. |
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The most prominent HMGB1 protein and mRNA expression arthritis is present in pannus regions, where synovial tissue invades articular cartilage and bone. |
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Effects of dietary mulberry, Korean red ginseng, and banaba on glucose homeostasis in relation to PPAR-alpha, PPAR-gamma, and LPL mRNA expressions. |
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In the present study, BPA transactivated the promoter sequence of c-FOS, EGR-1, and CTGF, and accordingly stimulated mRNA expression of these genes. |
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Expression of surfactant associated protein-A and Clara cell 10 kilodalton mRNA in neoplastic and non-neoplastic human lung tissue as detected by in situ hybridization. |
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Thus the origin of HBB mRNA is the erythroblasts in BM and reticulocytes. |
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Although SNP5 and SNP7 were intronic mutations, they also might affect alternatively spliced transcripts of mRNA or transcription factor binding, thus affecting phenotype. |
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Instead the nRNA product contains several short oligonucleotide sequences that are mirror images to unique sites on the viral mRNA from the viral replicative genes. |
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Therefore, we analyzed microarray-based transcriptome-wide mRNA expression profiles of the neuroglial cell line T98G after exposure to KJ and its single constituents. |
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