How to use incubated in a sentence

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Sentence Examples Four times PCV of buffer I was added and the mixture was incubated on ice for 20 min. Hepatocytes damaged by exposure to galactosamine, thiocetamide, and carbon tetrachloride were incubated with Picrorhiza constituents. The intact nuclei were incubated for 15 min after which they were gently pierced with two fire-polished glass micropipettes. The GA solution was dried in a rotor evaporator and then incubated under vacuum overnight in a reagent flask. Twenty microliters of each antibody was added to 100 L of blood, and the mixture was incubated at room temperature for 15 minutes. In a related experiment, articular cartilage was incubated for two days with the enzyme elastase, which specifically digests the collagen fibers. Pairs of test and control samples were incubated either in the darkness or under continuous illumination with white light. Other embryos were incubated directly in the Petri dishes for germination at a range of different temperatures. Leaf discs from plants expressing aequorin were excised and incubated in thiols as described above. Control cells consisted of untreated cells, cells incubated with the mannitol excipient and exposed to light or cells exposed to the dye alone. Incubators were set at the temperatures indicated, and in all cases plates at different temperatures were incubated in parallel. Wheat leaf segments floating on nutrient solution were incubated in Erlenmeyer flasks in permanent light. Fresh pullet eggs were incubated to obtain the primitive streak stage of development. To determine the presence of yeast in cloacae, swabs were streaked onto Sabouraud's dextrose agar and incubated for 14 days. The cultures were incubated at 37 deg C in the controlled microaerophilic atmosphere described earlier or three days. Fifty microliters of sarcosine were added and the cells were incubated on ice for an additional 15 min. It would therefore take a single female 40-50 days to lay the maximum clutch that could be incubated. We collected clutches, incubated the eggs, and took blood samples from hatching young. For immunocytochemistry, grids were incubated with the antibodies against macrophages. The embryos are incubated in the laboratory for an additional two to four days and transferred to the female partner's uterus. Show More Sentences The germinated seeds were planted in gravel and further incubated under culture room conditions. The homogenate was incubated at room temperature for 5 min with agitation and then supplemented with 6 ml of chloroform. Explants were incubated at different temperatures after bombardment and analysed for reporter-gene expression. Parentally incubated eggs were not infected, suggesting that incubation limits infection. After 24 and 48 h, aliquots were transferred to 96-well plates and incubated for 3 h with a tetrazolium salt. The slides were incubated for 30 min in a humidified chamber at room temperature. This hatchling is just one of hundreds of its kind that have been incubated and raised in captivity for release into North America's wetlands. Cells were then permeablized with saponin and incubated with fluorescent-labeled antibodies, and four-color flow cytometry was performed. Like the eggs of birds, monotreme eggs are incubated and hatched outside the body of the mother. Females built the nest and incubated alone, but both parents fed nestlings and fledglings. Slides were covered with a glass coverslip, sealed with rubber cement, and incubated overnight. Laid in burrows or under mounds, megapode eggs are incubated by the warmth of the sun, rotting vegetation, or volcanic vents. Radicles or plumules were excised from the embryos and similarly incubated. Cells were incubated in the presence of phorbol ester myristate to induce macrophage differentiation. Negative controls were previously incubated in 10 mM tropolone, a specific inhibitor for PPO activity. The inoculated cell cultures were incubated at 35C and observed daily for a week for cytopathic effect. The same preparation was readapted in darkness and incubated with an appropriate concentration of wortmannin, and the measurement was repeated. The eggs are incubated for 15 or 16 days and newly hatched troupials are fed equally by both parents. Innovation is running rampant in this whole arena, and new businesses are being incubated at a rate never seen before in the industry. The tissue was sandwiched between a positively charged glass slide and a siliconized coverslip and incubated for 30 min at room temperature. Show More Sentences Malignant cells incubated with nanoshells and exposed to the light source showed signs of irreversible heat damage and cell death, but control cells were left undamaged. Nutrients were thoroughly mixed into the substrate, water was added to field capacity and the pots were incubated for 10 d in a glasshouse before seeds were planted. Additionally, samples from the flocculent layer and from a semipurified bacterial mat were incubated under controlled oxygen conditions to determine production. In experiments that test the effect of actin, cells were incubated for 30 min before experiments in medium containing 20 M cytochalasin D to disrupt actin filaments. It didn't kill birds directly, but it did affect their internal calcium gradients so that all eggshells were too thin and collapsed when the parents incubated them. The chamber was incubated at 37'C for 1.5 h to allow gelation to occur. The leaves were inoculated with a droplet in which P. infestans zoospores were suspended and then incubated for 5 days. The only difference is that a bit of your scar tissue, which some wiseacre had placed in the deepfreeze will be taken out and incubated. The hybridization mixture was incubated at 90°C for 3 min and immediately put on ice. In ancient texts, basilisks are said to be strange hybrid creatures which come into being when chicken eggs are incubated by toads. When nornicotine, glucose and peptide were incubated together, the result was a modified peptide that had little tendency to agglomerate. Since the virus can stay incubated in the human body for up to 21 days, the third step, follow-up, is key. Eggs are taken from female salmon, fertilized with milt from males, and incubated under controlled conditions. You have incubated a lot and you have done a lot of brainstorming to innovate. The mixtures were incubated for another 2 hours, followed by adding different concentrations of hot water extracts to the triplicate culture wells. The supernate of SMC incubated with DMSA-MNPs under the same experiment conditions was collected and mixed. The frozen plasma was taken out from refrigeratory and incubated at 37 °C in a water bath until samples are completely thawed. Heparinized whole blood samples are incubated with different concentrations of the suspected allergen. Proteins such as fluorescently labeled DNA-binding transcription factors in solution are added to the chip and incubated on the array. Plates were incubated upright at 30°C for 48 72 hrs and were visually inspected for the formation of precipitin rings. Show More Sentences The mixture shall be incubated for one hour at 15 °C. This represents the inoculum. The inoculum culture shall be incubated under the same conditions as the test cultures. The bound microparticle complexes are then incubated with antidetection hapten alkaline phosphatase conjugate, which binds to the detection haptens. Such doolally customs were incubated no doubt in Gauloise-wreathed boardrooms in Paris decades ago. Oxidase test: With an inoculating needle, smear a portion of a colony from the recently incubated CCDA plate onto an oxidase strip. Plates shall be incubated at 14 ± 2 °C and examined microscopically for up to seven days. The explanted scaffolds were incubated with CFDA SE, followed by fixation with methanol and staining with propidium iodide. It was incubated by the philanthropic arm of Royal Dutch Shell, a giant oil company, but has since turned commercial. Two of the brainwaves of the 1990s welfare reform and zero-tolerance policing were incubated in conservative think-tanks. Mastodon C is one of four infant firms being incubated under the ODI's roof. In this section Where are the songs of spring? In addition, individual bacterial isolates from the tank mixes and leaf washings were incubated in the presence of the late blight pathogen. The young of most reptiles hatch from eggs that are incubated in sand, moss and other substrates. First, the milk is fermented through inoculation with the bacterial culture and then incubated at an appropriate temperature. Note that if the eggs have already been incubated by the parent bird the number of days should be reduced accordingly. To optimise the hatching results of artificially incubated eggs, you will need some experience in the careful operation of the device. Whereas adults and eggs are mainly sold for human consumption, some eggs are incubated and hatchlings are exported to international pet markets. The egg is relatively large, weighing about 100 g, and is incubated, or kept warm, continuously by one of the parents. While the virus can remain incubated for up to 21 days, it is not contagious until a patient begins showing symptoms. The plates were incubated until the colonies were easily countable. All adults at the nest incubated eggs and brooded the naked chicks. Show More Sentences After incubation for half an hour, the sections were rinsed with tris buffer, peroxidase solution was added, incubated for 30 min and later rinsed with the buffer. The malt is then boiled, strained, and incubated with yeast. The formation of this peak increases with irradiation time when the mitochondria are incubated under control conditions and present high membrane potentials. The incubated SF was rinsed twice with distilled water before further submersing. Catskill refugees, they were tummlers and shpritzers incubated in resorts, supper clubs, casinos. They are dipped into the water and incubated for 48 hours. After cooling on ice, the samples were incubated with 30 mM iodoacetamide for 1 h in the dark to carbamidomethylate the cysteine residues. The eggs are incubated for 19 to 25 days, the chicks can fly after 12 to 13 days after hatching and are fully grown after 30 to 35 days. Punches were placed in haemolysing reagent, in duplicate or triplicate, and incubated at room temperature with shaking. All antral follicles were aspirated, and cumulus-oocyte complexes and naked oocytes were incubated for up to 48 hours in IVM culture media. Mated pairs produce one to three eggs per year, laid two to five days apart in March or April and incubated for 38 days by both parents. It will be exciting to continue to learn from their experiences and to see what the young graduates incubated in this inspiring environment can accomplish. Of the 10 chicks hatched from artificially incubated eggs, one 5week-old, hand-fed, Indian ring-necked parakeet had a positive cloacal swab. Statistics Canada states that a high proportion of incubated companies survive the critical early years, whereas most companies that go it alone, fail to make it past their first birthday. It is a model of how effective responses to the dilemmas of the Canadian food system can be stimulated, incubated, managed, and co-ordinated by local people. A remarkable increase in the pHi sensitivity of voltage-dependent calcium channels occurs in human sperm incubated in capacitating conditions. They did that because there was clear evidence that the parasites and diseases being incubated on these farms were being transmitted to both smolts leaving the river mouths and to wild mature salmon returning to spawn. The smear was then rinsed in tap water, and incubated in malachite green solution for 9 sec. The centre has since incubated over 100 companies. For some experiments, DCs were incubated with biopharmacological inhibitors to various molecules. Show More Sentences If cytotoxicity occurs after seven days, subcultivation shall be performed once and the cells shall be incubated to achieve the total of 28 to 36 days incubation from the primary inoculation. Tubes of these semi-solid media can be incubated aerobically. After incubated for 2h, the purpurogallin formed was extracted with 35 mL ether and determined by taking the absorbancy at 420 nm. In order to boost the immune system, vitamin D3 and natural or synthetic curcumin were incubated with macrophages exposed to beta-amyloid. The wort is incubated with brewer's yeast, which secretes the enzyme maltase to convert maltose to glucose and the enzyme zymase to convert glucose to ethanol. In one experimental study, infective larvae incubated in vitro with blood cells were administered intra-abomasally to see their establishment potential. Founded in 2010 by Nikhil Sethi and Garrett Ullom, Adaptly was incubated through DreamIt Ventures and is now based in New York City. After two weeks, mycelium growing from the edge of the periostracum and free swimming flagellates, possible fungal zoospores, were observed in the culture incubated at room temperature. Their agonistically incubated works are synopsised, shrivelled. To test for macrocyst formation, we incubated each clone both by itself and with each other clone tested under conditions favorable for macrocyst formation. When not being incubated the eggs are covered with sodden debris. The drab green or grayish olive eggs must be incubated for 24 to 28 days. Agar is a substance that is used in the culture of bacteria and other microorganisms, used mostly used in laboratories, For instance, petri plates are lined with agar gels and incubated. Besides the aforementioned carbohydrates, we also observed inhibition of the hemagglutination when we incubated the lectins with fucose. The eggs are incubated mostly by the female for 31 to 32 days. The nest is a shallow scrape on the ground lined with vegetation, into which typically four eggs are laid and incubated by the male and female parents. Synchronized cultures of P. falciparum trophozoites were grown in human RBCs and incubated with increasing concentrations of purified human platelets. All bottles were incubated at a constant mesophilic temperature. Reconstituted sweet WPC80, cream powder and an emulsion of anhydrous milk fat and demineralized whey were incubated with porcine pancreatic lipase at 37 C for 18 hours. A 2009 study in the journal Science suggested that it was males of the small, carnivorous dinosaurs Troodon and Oviraptor that incubated their eggs. Show More Sentences The cocultures of HeLa cells and lymphocytes were incubated for 24 hours. If there is no antibody present, an immediate spin crossmatch or computer assisted crossmatch is performed where the recipient serum and donor rbc are incubated. The eggs are incubated by the females by day and by the males by night. Examples from Classical Literature The eggs which have been incubated to an advanced degree are as eagerly devoured as those quite fresh. These loaves are incubated for about a month at room temperature. They kidded him into thinking that he had incubated a Whale. Putrefactive bacteria, for example, were incubated for an hour with the artificially produced antimicrobial peptides. In a similar experiment, the samples spiked with morphine and M3G were incubated for 0 and 12 h under the same conditions. At that time, four incubated ICT companies were presented to King Abdullah II and to participants at the forum. Over the past 15 years Primo Medical Group has incubated and invested in several new technologies including Angiolink Corporation, Arthrosurface Inc. At the same time Working Capital has tested some 6,000 business ideas, incubated and funded 19 start-ups, pre-incubated 36 start-ups and assigned 109 grants. Instead of passing out of the hen's body and being incubated outside, the egg was incubated in the hen for 21 days and then hatched inside the hen, the BBC reported. Find more words! 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What is the opposite of incubated? Words that rhyme with incubated What is the adjective for incubated? What is the noun for incubated? Use our Sentence Generator Nearby Words incubate incrusted incrustation incrust incriminatory incrimination incubates incubating incubation incubation period incubations incubator 9-letter Words Starting With i in inc incu incub incuba incubat incubate