How to use electrophoresis in a sentence

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Sentence Examples The proteins in the immunoprecipitates were size fractionated by SDS polyacrylamide gel electrophoresis. The gel was stained after electrophoresis with ethidium bromide and photographed. For gel blot analysis, 10 g of total RNA was applied to each lane and separated by electrophoresis. Fleischer used gel electrophoresis to discriminate yolk allozymes sampled from eggs laid by several female cowbirds. Charged colloidal particles will migrate in an applied electric field, a process called electrophoresis. For this study, electromorphs were typed by agarose gel electrophoresis or isoelectric focusing. The purity of the double-stranded oligonucleotides was confirmed by polyacrylamide gel electrophoresis. In the final step, the investigator cuts plugs to fit the wells of an agarose electrophoresis gel. After electrophoresis, the agarose gels were stained with ethidium bromide and visualized by ultraviolet light. During conventional electrophoresis, molecules larger than 50 kb have essentially the same motility and remain unseparated. As it can be seen on the electrophoresis gel, the frank scission is highly selective. The use of polyacrylamide gel electrophoresis also can be used to determine the isoelectric charge of proteins. First, after electrophoresis of the proteins through a polyacrylamide gel, they are transferred by blotting to a porous membrane sheet. All isolates were subjected to molecular typing by pulsed-field gel electrophoresis. The first was protein analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis. The purified protein migrated with an apparent molecular weight of 42 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Polymerase chain reaction products were examined by agarose gel electrophoresis and ethidium bromide staining. Each step in the curing procedures was monitored by electrophoresis of plasmid isolations. The purity of enzyme preparations was analysed by gel electrophoresis and spectral analysis. The results of liver tests were normal, as were those of a chemistry panel and hemoglobin electrophoresis. Show More Sentences They separated the DNA out of the blood and analyzed it with conformational gel electrophoresis. After electrophoresis, the proteins were transferred electrophoretically onto a nitrocellulose membrane as described previously. Appropriate hemoglobinopathy screening should include a complete blood count and hemoglobin electrophoresis. Dyck and Craig employed capillary electrophoresis as the medium for single-molecule enzyme isolation. The triplex and biplex PCRs were combined before electrophoresis and loaded onto a single gel lane to form a pentaplex system. All oligos were obtained from Sigma Genosys and further purified using polyacrylamide gel electrophoresis. The presence of a dicentric chromosome was confirmed by Southern blot analysis of orthogonal field alternating gel electrophoresis gels. Following electrophoresis, the gels were examined under UV light transillumination. After centrifugation, the cell pellet was processed for microgel electrophoresis to determine sensitivity to mutagens. Protease samples were submitted to electrophoresis in polyacrylamide gels under denaturing conditions, according to Laemmli. The immunoglobulin type can be determined by immunoelectrophoresis or immunofixation electrophoresis. Its purity and integrity was assessed using agarose gel electrophoresis. Except for the lowest molecular weight samples, all of the average molecular weights were determined by agarose gel electrophoresis or viscometry. The intactness of RNA was monitored by agarose gel electrophoresis. Our buffers and detergents can handle your most challenging electrophoresis applications. An initial preference for electrophoresis seems to be shifting to probes, as these improve in quality. Since DNA molecules have much higher molecular weights than proteins, the molecular sieve used in electrophoresis of DNA must be different as well. This is because separation according to the isoelectric point and the molecular weight are carried out through time-consuming gel electrophoresis analysis. Instead, it generated a series of DNA molecules of varying lengths that could be separated by using polyacrylamide gel electrophoresis. After electrophoresis, the gel was stained with CBB for 2 h and was destained. Show More Sentences Falsification of milk can be detected chromatographically or by electrophoresis. Her peripheral blood smear, serum protein electrophoresis, and urine immunoelectrophoresis for Bence-Jones protein were normal. The size and purity of the amplified product were checked by agarose gel electrophoresis, and the fragment was purified from the gel by commercially available procedures. These include the single cell gel electrophoresis assay, the gel electrophoresis assay, the alkaline elution and unwinding assays and the analysis of DNA adducts. It involves a lot of different sciences-molecular biology, human population genetics, some aspects of chemistry electrophoresis, etc. The PCR products were analyzed by electrophoresis on agarose gels containing ethidium bromide and were photographed under ultraviolet transillumination. The mobility decrements may be thought of as shape factors characterizing the apparent curvature of a target fragment, as determined by gel electrophoresis. Two-dimensional gel electrophoresis is one of the most powerful resolving methods now available. Screening should consist of a complete blood count, as well as hemoglobin electrophoresis or hemoglobin high performance liquid chromatography. By taking advantage of electrophoresis technology, the Needle Free mesotherapy equipment enable the cell membrane to appear micropores. After the electrophoresis field is cancelled, the micropores will disappeared and there won't be any negative influence to the cells. Slurry samples were periodically taken from the microcosms for analysis of community rDNA using denaturing gradient gel electrophoresis. Thymidine kinase DNA of TK6 human lymphoblast cells was analyzed using electrophoresis. Last month's Primer dealt with endpoint product detection methods for PCR, with a primary focus on the agarose gel electrophoresis method. Altogether 12 saccharides and sugar alcohols were identified using capillary electrophoresis. Patterson, University of Texas by Ca3 probe, contour clamped homogeneous electric field karyotype, and CHEF pulsed-field gel electrophoresis. Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis. The macrocyclic antibiotic vancomycin has been used as a powerful chiral selector, specifically in capillary electrophoresis. A quick method for the simultaneous determination of ascorbic acid and sorbic acid in fruit juices by capillary zone electrophoresis. Thousands of samples can now be sorted by this PCR alone without needing Southern blot or capillary electrophoresis. Show More Sentences We measured FLCs and TUP in 105 urine samples on which immunofixation electrophoresis had been performed. By utilizing the electrophoresis technique, F-49B Needle Free mesotherapy equipment can open the aquaporin easily and send nutrition into deep-seated skin layer. By making use of electrophoresis technology, this needle free mesotherapy equipment can easily open the aquaporin, and then send the nutrition and active substances, etc. into deep-seated skin layer. These costs include sample preparation such as the destaining of the electrophoresis gel, tryptic digestion and extraction of the peptides from the gels. Detection of oligoclonal bands in cerebrospinal fluid by immunofixation electrophoresis. The following Annex contains a list of characteristics based on isozyme markers revealed by electrophoresis and a description of the method to be used. Serum protein electrophoresis showed a polyclonal hypergammaglobulinemia. Serum protein electrophoresis showed polyclonal hypergammaglobulinemia. The protein gels were fixed using destaining solution immediately after electrophoresis and the protein bands were visualized under UV light. The result of the PCR is checked by electrophoresis after hydrolysis by two sets of restriction enzymes, making it possible to check the presence of both the GFP insert and the mutation. These DNA's, subjected to several restriction enzymes, show characteristic profiles after electrophoresis, specific to the varieties of the organism from which they originate. When gel is polymerised, combs are removed and gel is placed in a horizontal electrophoresis system containing enough 1 X TAE to the cover agarose gel. When the agarose gel has set, remove the comb from the tray, place the tray with gel in the electrophoresis apparatus and fill reservoir with 0.5 X TBE buffer to cover gel with buffer to a depth of 4 mm. Molecular subtyping was performed by ApaI and SmaI pulsed-field gel electrophoresis and enterobacterial repetitive intergenic consensus. The research team has been using twodimensional polyacrylamide gel electrophoresis for comparison of culture conditions that influence protein expression of F. psychrophilum. It takes the protein sample isolated by electrophoresis and integrates a number of procedures that must be conducted before the sample can go to a mass spectrometer. Fortunately, it is just starting to acquire it. Spot the differenceGel electrophoresis works by pulling protein molecules through a gel using an electric field. In contrast to electrophoresis, this method has been applied to a wide variety of biological compounds and has contributed enormously to research in biochemistry. Each fraction was then characterized and quantified by agarose gel electrophoresis and brevetoxin radioimmunoassay, respectively. The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining. Show More Sentences Genetic variation was screened by cellulose acetate electrophoresis for 991 saugers in 11 populations from the mid-west and the Great Plains. Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons. Gel electrophoresis patterns showed that multiple freeze-thaw cycles could cause cross-linking of protein in the myofibrils of muscle cells. Electropherogram generated by capillary gel electrophoresis with phospholipid nanogel matrix shows separation of amplicons over time. High-performance electrophoresis elimination of electroendosmosis and solute adsorption. Analytical methods include titration, liquid chromatography, gas chromatography, ion chromatography, capillary electrophoresis and potentiometry. That was a wonderful opportunity because I could see the patient and then do the electrophoresis and immunoelectrophoresis in the laboratory. Serum protein analysis still involves agarose gel electrophoresis and gel-based immunotyping for gammopathies. Separation of nicotic acid and its structural isomers using 1-ethyl-3-methylimidazolium ionic liquids as a buffer additive by capillary electrophoresis. It was then digested with restriction enzymes, separated using gel electrophoresis, and transferred to a nitrocellulose membrane by a Southern blotting mechanism. Other important diagnostic tools of clinical chemistry including the methods of spectrophotometry, electrophoresis and immunoassay were first devised by Westerners. Genetic analysis methods like ribotyping, pulse field gel electrophoresis and multilocus sequence typing have shown additional discriminatory power over serotyping. Detection of in vivo genotoxicity of haloalkanes and haloalkenes carcinogenic to rodents by the alkaline single cell gel electrophoresis assay in multiple mouse organs. Total plasma protein concentration, plasma hemoglobin concentration, and plasma protein electrophoresis patterns were determined for both hemolyzed and nonhemolyzed samples. Capillary electrophoresis is a technique which has nothing to see with chromatography, but which is based on the mobility of ions in an electric field. The eluates were collected by centrifugation, washed 3 times with 1x denaturing elution buffer, and examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The subunits are connected via an interchenar disulfide bond which can be easily broken in the absence of reducing agents as shown by SDSPAGE electrophoresis. Determination of genome size, macrorestriction pattern polymorphism, and nonpigmentation-specific deletion in Yersinia pestis by pulse-field gel electrophoresis. To identify the allergen in the cyanobacteria that is binding to IgE, we performed 2D gel electrophoresis, followed by immunoblotting, using MC lysate and pooled patient sera. The slideshow, which should take no more than 12 minutes to present, first describes how DNA is restriction digested and separated by size using agarose gel electrophoresis. Show More Sentences The ability of glutaraldehyde cross-linked DNA condensates to protect DNA from sonicative destruction in the presence of sodium chloride was evaluated by gel electrophoresis. Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis. Originally, the laboratory tested for immunoprotein levels and conducted tests for autoimmune disease by electrophoresis, serological techniques, and radiochemical techniques. The immunoprecipitated samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes. Examples from Classical Literature Java of R-type ACSSpSuT from patients in England and Wales and Scotland were characterized by phage typing, plasmid profile typing, and pulsed-field gel electrophoresis. Find more words! 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What is the adverb for electrophoresis? What is the adjective for electrophoresis? What is the noun for electrophoresis? Use our Sentence Generator Nearby Words electrophoresed electrophorese electrophilic electrophile electro-osmotic electro-osmosis electrophoretic electrophoretically electrophoretogram electrophorus electrophotography electrophysiologic 15-letter Words Starting With e el ele elec elect electr electro electrop electroph electropho electrophor