| Following a potential recombinant amplification phase, a marker rescue assay cell line is transduced with the test batch. |
| Cells transduced with a vector batch produced with same system. |
| The impulses are relayed by the few remaining retinal cells and are transduced as normal to the optic nerve pathway, resulting in vision. |
| A proportion of the transduced cells is infected with several dilutions of an RCL-positive, in order to define the assay threshold. |
| The number of cells in the PCR wells is determined by measuring the copy number of an endogenous gene from the transduced indicator cells. |
| Or on target cells: a PCR reaction is performed on genomic DNA extracted from target cells transduced with the vector batch to be tested. |
