| Sequencing of sporangiospore cDNA clones was carried out in both directions at the University of Washington on commercial contract. |
| Ac2VRR-RXL-65 has an ORF of 450 bps encoding a secreted protein of 17.4 kDa, with low-level expression detected by 1 clone in infected seedling library, and 2 clones in the sporangiospore library. |
| Use of enzymes to degrade the ungerminated sporangiospore cell walls, or of the cell walls of germ tubes from germinating spores, appeared to be one of the methods of choice. |
| Sporulation on Czapek agar enabled preparation of a sporangiospore suspension used for antifungal susceptibility testing. |
