The lysed cell preparation allowed exposure of the FRETbased sensor to known cAMP concentrations and measurement of FRET signals using a spectrofluorimeter. |
Hydroxyterephthalic acid is a highly fluorescent molecule and it was detected by HPLC-FLD or spectrofluorimeter. |
Perkin Elmer LS 55 spectrofluorimeter was used to obtain the fluorescence spectra of the fluorescence material. |
The emission spectra were measured using a custom-made calibrated spectrofluorimeter described previously. |
Skin hypericin content is determined through fluorescence emission with a fiber optic attachment to a spectrofluorimeter. |
Activity was measured using a Victor 2 spectrofluorimeter. |