In addition, phalloidin staining of the actin cytoskeleton and propidium iodide uptake assays were performed to investigate cellular structural integrity. |
Before analysis, cells were spun out of ethanol and resuspended in 1.5 ml PBS containing propidium iodide and RNase. |
Three common flow cytometry dyes are fluorescin isothiocyanate, phycoerythrin, and propidium iodide. |
We measured cell cycle distribution and apoptosis using propidium iodide staining and a flow cytometer as described previously by Mandil et al. |
Supravital exposure to propidium iodide identities apoptotic cells in the absence of nucleosomal DNA fragmentation. |