| For extinction coefficient measurement, pyridine hemochrome spectra were acquired in triplicate, from 600 to 500 nm in 1 nm steps, using a 1 s averaging time per step. |
| The characteristic spectrum of hemochrome was not detected in any sample after reduction. |
| The heme b content was quantified by the pyridine hemochrome method. |
| We also monitor temperature, heart rate, mean blood pressure, hemochrome, blood gases, blood lactates and SOFA score. |
| The concentration of the holoprotein was then determined by the pyridine hemochrome assay using an ε418 extinction coefficient of 191.5 mM 1 cm–1 as previously described. |
