| Rumen fluid was used for direct counts of bacteria, protozoa and fungal zoospores using methods of Galyean by haemacytometer and counted under a electromicroscope. |
| The ratio of the number of vital and dead cells in the suspension was obtained by staining the cells with trypan blue dye and counting in a Neubauer haemacytometer. |
| The cells were then examined by trypan blue exclusion in a haemacytometer. |
| The spore count was done using haemacytometer and the concentration was adjusted to 107spores per mL before it was kept in the cold room for further used. |
| The total direct count of protozoa was made by the methods of Galyean based on the use of a haemacytometer. |
| Cell counts were performed using a Neubauer haemacytometer and cell viability determined by neutral red uptake. |
