No. 2 and having an exonuclease activity removed by replacing the aspartic acid residue at position 8 with an alanine residue. |
Phage gene 46 encodes one subunit of a putative exonuclease that is thought to be directly involved in recombination and recombinational repair. |
Orn was shown by in vitro analysis to be an exonuclease specific for RNA molecules shorter than five nucleotides in length. |
So Oxford Nanopore has also been looking at an alternative approach, called exonuclease sequencing, in conjunction with Illumina, an American firm that is the market leader in rapid sequencing. |
The presence of the mismatched nucleotide greatly inhibits the addition of the next nucleotide, and the pause in the polymerization process gives the exonuclease time to remove the noncomplementary nucleotide. |
Data from 4 additional polymerases could not be analyzed because the template was degraded by 3' to 5' exonuclease activity before acquisition. |