| At steady state, cells were centrifuged and the fluorescence of the supernatant measured. |
| The 10 ml samples were centrifuged and the absorbance of the supernatant was measured at 280 nm to determine the lignin content. |
| After 10 s mixing with vortex, the suspension was centrifuged at 16 000 g for 2 min. |
| After cooling to room temperature, the solution was centrifuged at 800 rpm for 5 min. |
| Bile must be centrifuged and examined under polarizing or light microscopy for detection of precipitates. |
| These were shaken for 1 hour, then the solutions were centrifuged and the clear supernatant was pipetted into vials. |
