A unit of packed red blood cells aliquoted into two bags was ordered for transfusion. |
The suspension was vortexed for 5 minutes, sonicated for 30 seconds and aliquoted into 100-litre samples. |
The IP pellet was resuspended in final wash buffer and aliquoted for subsequent biochemical analyses. |
Where appropriate a master mix of PCR reaction components was made and aliquoted into individual reactions. |
After 10 min of incubation at room temperature, 1.25 ml of solubilized extracts were aliquoted into 1.7-ml microcentrifuge tubes. |
From each patient, 6-10 ml of venous blood was drawn and aliquoted in plain tubes without anticoagulant. |