Thirty microlitres of PCR product was loaded on 1.5 per cent agarose gel electrophoresed and stained with ethidium bromide. |
For all 43 species, amplified fragments were purified from agarose gels and ligated in the TOPO TA cloning vector. |
Somatic embryos at late developmental stages were gently extracted from agarose layers with forceps. |
For this study, electromorphs were typed by agarose gel electrophoresis or isoelectric focusing. |
The vesicles filled with agarose gel and sucrose solution were diluted into an isoosmolar glucose solution. |
The digest was fractionated overnight on an agarose gel as described above. |