The absorbance measured is directly proportional to the concentration of human leptin present. |
The absorbance was always kept below 0.05 to avoid self-absorption of the fluorescence. |
The 10 ml samples were centrifuged and the absorbance of the supernatant was measured at 280 nm to determine the lignin content. |
The absorbance was measured with a microtiter plate reader at a test wavelength of 405 nm. |
First, the emission spectrum of the donor molecule must overlap with the absorbance spectrum of the acceptor. |
Upon measuring the absorbance of a labeled peptide at a given angle of incidence, two different dichroic ratios were obtained. |