Superoxide anion was estimated by spectrophotometric measurement of the reduction of nitro blue tetrazolium. |
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The standard procedure for this assay was to remove the cell culture medium and add fresh media containing the MTS tetrazolium reagent. |
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The tetrazolium ion, which is commonly used in viability tests, could not penetrate the inner coat of watermelon, tomato or pepper seeds. |
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The ability of the seedlings to reduce triphenyl tetrazolium chloride was then determined, using the method of Steponskus and Lanphear. |
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After 24 and 48 h, aliquots were transferred to 96-well plates and incubated for 3 h with a tetrazolium salt. |
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The stains acridine orange, ethidium bromide, azur II, eosin B, trypan blue, Giemsa solution and nitroblue tetrazolium were purchased from Sigma. |
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Seeds are soaked overnight in water then treated with tetrazolium to give an indication of viable, abnormal, and dead seeds in the seed lot. |
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Upon exhumation, we examined each replicate for germinants, rotted seeds, and whole firm seeds, which we tested for viability with tetrazolium. |
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The colonies grown on Compact Dry TC are red due to redox indicator tetrazolium salt. |
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This technique is more reliable than tetrazolium chloride for this species, because some deeply dormant viable seeds show minimal staining with tetrazolium. |
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On Kelman's tetrazolium and SMSA media, the whorls are blood red in colour. |
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The hearts were then stained with tetrazolium and infarct size determined with planimetry. |
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A method of steam sterilization of an article, which method comprises exposing to steam both said article and a steam sterilization indicator, said indicator comprising, as active ingredient, a tetrazolium salt. |
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Contains: tetrazolium salt, citrate buffer. |
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The MTS tetrazolium compound is bioreduced by cells into a colored formazan product that is soluble in tissue culture medium. |
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