| The investigators derived the protoplasts from GAS by treating the GAS with phage lysin in a hypertonic medium, to prevent lysis. |
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| Solubilization of the cell wall with phage lysin also yielded a large M-protein molecule. |
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| Evidence that PP may play a dominant role in UV-induced transition mutagenesis has been obtained for bacteria and lambda phage. |
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| One hundred thousand unamplified phage were rescued with Exassist helper phage in SOLR cells to generate a library in pSKII for EST sequencing. |
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| These phage do not block superinfection, and recombination mediated by host enzymes can occur in multiply infected hosts. |
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| Positive phage clones were converted to plasmid vectors using the material provided in the cDNA synthesis kit. |
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| The fitness component of phage growth rate was measured to quantify this phenomenon. |
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| The deletion affected gene yajF with unknown function, but associated with genes involved in phage resistance through abortive infection. |
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| Progeny phage that produced clear plaques in the presence of IPTG and X-gal were analyzed by a differential oligonucleotide hybridization technique, as reported. |
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| The MRSA and MSSA had identical ribotypes and a rare phage type not recorded previously by the Dutch reference center. |
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| Wild ones cannot. Dr Farah's team began work with 300 variegated samples of their target, and 25 strains of an appropriate phage. |
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| Bacterial viruses are called phages, and phage therapy, while about a century old, is making a bit of a comeback, perhaps as a substitute for antibiotics. |
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| Recombination can occur between exogenous DNA introduced into bacteria by conjugation, phage transduction, or DNA transformation and the bacterial genome. |
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| In contrast to the phage polymerases, RNA polymerases from higher organisms have a much more complex subunit structure than the bacterial enzymes. |
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| This phenomenon is similar to suicidal defense against phage infection, or phage exclusion, programmed by prophages and plasmids, and may well play a similar role. |
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| Specialized transducing phages generated from the prophage by illegitimate recombination usually contain the E. coli genes gal or bio that are adjacent to the phage genome. |
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| The computer phage really replaces the executable file with its own code instead of appending to it. |
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| Drug discovery and new therapeutics including alternates to antibiotics such as probiotics and phage therapy. |
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| Unlike antibiotics, the potential for bacteria to develop resistance to phage endolysins is thought to be much lower. |
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| It is recommended that the selected isolates of S. Enteritidis and S. Typhimurium are phage typed. |
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| Plates can be examined for lysis at 24 and 48 h after inoculation with phage. |
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| The DC1 phage or enzyme could be used together with other phages or antibiotics to eliminate BCC more effectively. |
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| The efficacy of the isolated phage to disinfest seed potato tubers artificially inoculated with a common scab-causing streptomycete was evaluated. |
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| He and his colleagues began working with a phage that infects the anthrax bacterium almost exclusively and identified the gene encoding its lysin. |
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| The prophage DNA is then excised from the bacterial chromosome, and the phage replicates, producing many progeny phages and lysing the host bacterial cell. |
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| The bacteria that carry the noninfective precursor phage, called the prophage, remain healthy and continue to grow until they are stimulated by some perturbing factor, such as ultraviolet light. |
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| The selected phage clones can be affinity maturated to improve their sensitivity and genetically engineered to be employed in different assay formats. |
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| But at least by then I had accomplished my summer lab objective of showing that peroxide-treated phages had biological properties identical to those killed by X-ray-irradiated phage lysates. |
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| They then destroy, or lyse, the cell, releasing new phage particles. |
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| As is typical of phage, Tsamsa only infected a small group of closely related bacteria, showing a slight preference for B. anthracis. Like all temperate viruses, Tsamsa exhibits a Jekyll-or-Hyde life cycle. |
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| Within half an hour, the bacterium explodes under the pressure, releasing the phage progeny into the surrounding area, where they lie dormant until their next meal comes along. |
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| The phage display technique has wide application, and it is recognized that retaining rights to practise a technology in a single entity would considerably slow the progress of biological research in general. |
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| This new method, introduced in 2008, is based on the latest technology available for food pathogen screening: the recombinant phage protein, which offers unique specificity and sensitivity. |
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| Experiments with phage therapy began more than 80 years ago in Russia, but did not attract attention in the West until more recently. The unfortunate zebra's phage, named Tsamsa, is a member of the Siphovirus family. |
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| Such a virus particle is called a transducing phage because, when it infects a bacterial cell, it can transmit the gene captured by λ phage DNA into the next bacterial cell it infects. |
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| Complementary to rational design, a phage library was constructed to explore further the residue requirements at the P4, P3 and P3' sites for multivalent and optimized bridge-binding. |
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| Panning of the phage library has led to thrombin-inhibitory peptides possessing strong anti-clotting activities in the low nanomolar range and yet interfering only partially with the catalytic active site of thrombin. |
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| The scientists are recognized for the development of phage recombinant proteins, which offer best-in-class specificity and sensitivity for the targeted capture and detection of bacteria from a sample. |
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| The host-binding domain of the P2 phage tail spike reveals a trimeric iron-binding structure. |
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| The source of the sdAbs is a newly constructed, high quality, phage display library comprised of the variable domains of the heavy chain antibody repertoires of a camel, a llama and an alpaca. |
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| Toward the end of the 1930s, Max Delbrück had the intuition that the question of autocatalysis, that is, replication, could be attacked through the study of phage, i.e., viruses replicating in bacteria. |
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| Lysogenic conversion by a filamentous phage encoding cholera toxin. |
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| We examined the efficiency of plaquing and phage virion integrity, as determined by in vitro assembly, immunological detection and electron microscopy. |
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| They also determined the gene coding for the phage endolysin that's responsible for degrading cell walls before the release of certain virus particles known as virions. |
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| Phage gene 46 encodes one subunit of a putative exonuclease that is thought to be directly involved in recombination and recombinational repair. |
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