| Primary sporocysts were obtained after overnight axenic culture of miracidia as described. |
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| Generally, host seeking by miracidia includes a complicated behavioral process that is initiated through a combination of light and gravity stimuli. |
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| Chemicals, usually in the form of short-chain fatty acids, or specific amino acids, which are released from the molluscan host, then attract the free-swimming miracidia. |
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| Four hundred and fifty six molluscs were individually exposed to one miracidia. |
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| Total RNA was isolated separately from miracidia, sporocysts, cercariae, schistosomulum, and male and female adult worms. |
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| Neither SjIR-1 nor SjIR-2 was expressed in measurable levels in sporocysts or miracidia. |
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| The effect of infection on snail fecundity and fertility, growth rate and survival was studied during the 62 days following miracidia exposure. |
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| Whereas SmHox8, SmHox1, and Smox1 were all strongly expressed in eggs and miracidia compared to other stages, SmHox1 was much more weakly expressed in all the other stages. |
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| Not only do P. sinitsini become adults in E. symmetrica, but eggs are produced and hatch inside the daughter sporocysts, releasing free-swimming miracidia. |
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| It was not only possible to generate mother sporocysts from miracidia in vitro, but also to obtain adult worms from cercariae through in vitro cultivation. |
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| We found that increasing the number of hosts sampled, rather than the number of miracidia per host, gives more robust estimates of genetic diversity. |
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| In addition, the ovicidal action of PZQ caused inviability of eggs by retraction of miracidia, mostly of mature eggs. |
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| Miracidia will then develop into sporocysts and produce cercariae. |
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