How to use cell culture in a sentence

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Sentence Examples A single cell culture, based on protoplasts that were isolated from the leaf mesophyll of tobacco, was used. The standard procedure for this assay was to remove the cell culture medium and add fresh media containing the MTS tetrazolium reagent. Ciprofloxacin treatment is considered as a safe and effective method for elimination of cell culture mycoplasmas. They also used a cell culture model and performed genetic analysis in human patients. This is based on their ultrastructural, cell culture, and immunohistochemical features. The dynamic stimuli of active cell culture systems promote special responses and phenotype expressions. His inventions include an electronic microinjection device and a flexible substrate for cell culture. Any cell biologist will tell you that the matrix a cell grows in is one of the fundamental variables of cell culture. In plant cell culture, the addition of exogenous sugar to the culture medium is an absolute necessity for nearly all tissues. The mesodermic cell culture consisted of a variety of cells, each capable of differentiating into specific types of daughter cells. In vitro studies involve exposing cells from the same cell culture and with the same genetic characteristics to a chemical or physical agent. A group of nerve cells derived from a cell culture that can be used for experimental purposes. Roberta Brinton, at the University of Southern California, has succeeded in growing neurons in a cell culture on electronic chips that can intercommunicate. Precipitating antigen is prepared in any cell culture system that supports the rapid multiplication of a reference strain of bluetongue virus. Concurrently, Celmed acquired the shares of two California companies that developed a neural cell culture and transplant technique. The facility will be used for the production of monoclonal antibodies and recombinant proteins based on mammalian cell culture technology. Autolysed samples can be cytotoxic to the cell culture and consequently limit its use. Oxygenation of culture media is achieved by the double-layer gas perfusion channel structure along the cell culture region. A double-layer gas perfusion network structure fabricated above the cell culture region was designed for parallel mammalian cell culture. You state here the possibility of being able to undergo continuous cell division for years in cell culture. Show More Sentences The cell culture fluids should be assayed for infectious virus, using an appropriate susceptible host. Cytogenetically, cell culture procedures and PGA fibrin scaffolds did not significantly alter chromosomal integrity of the chondrocyte genome. Thes animal models will be used to identify new therapeutic targets that will be confirmed in cancer cell culture models. Green tea has shown remarkable anti-inflammatory and cancer chemopreventive effects in many animal tumor bioassays, cell culture systems, and epidemiological studies. To summarize, metaphase spreads were made from a 24-hour, unstimulated cell culture and were G-banded by the standard trypsin-Giemsa banding method. The first breakthrough in the development of insect cell culture was the report of maturation of testicular follicle cells to spermatocytes in a moth in an artificial medium. The platelet yield obtained by applying this cell culture method is therefore improved. Initial pathology results were negative, but after additional days of cell culture growth, mild cytopathic effects were evident. More recently, in 2011, Sasai's team reported that they had used their cell culture system to grow partial pituitary glands. This cell culture technology can be used for faster and more flexible manufacturing start-up in a pandemic. The heterotypic cell culture system promotes cell cell interactions maintaining a harmonized physiology. This was shown in cell culture analyses, in animal studies and in theoretical arguments based upon its known physical properties. Between removing the donor cell and fusing its nucleus with an enucleated oocyte, there is a cell culture phase. Scientists have developed a three-dimensional brain cell culture system that resembles brain tissue in many of its characteristics. The segments of the cell culture chamber were filled with 100 μl cell culture medium. Genetic studies, ion channels, molecular signaling in cardiology, cardiac development, and cardiac tissue cell culture. Differentiation of vertebrate skeletal striated muscle can be studied in cell culture and provides a valuable model system for cell differentiation. Use of a polycrystalline porous material, consisting essentially of aragonite, as a three-dimensional solid support for in vitro cell culture. They will also develop a CF cell culture facility and make it available to other investigators who need cells for their research projects focused on cystic fibrosis. Secobarbital attenuates excitotoxicity but potentiates oxygen glucose deprivation neuronal injury in cortical cell culture. Show More Sentences This result indicated ADSCs are alive after bioencapsulation but a possible cell loss might happen in a longer in vitro cell culture. Two-dimensional human cell culture models of HCM are species-specific but lack biofidelity and do not measure contractility. Very recently, graphene provides a novel substrate for cell culture. This project breaks new ground in vaccines technology by demonstrating effective large scale production capacity using cell culture on a commercial scale. In addition, and increasingly, there are new studies published of animal and cell culture experiments that show alarming levels of genetic damage following exposure to uranium, depleted or not. Boehringer Ingelheim has the worlds largest capacities for contract development and manufacturing of mammalian cell culture products and is Europe's leading manufacturer of biopharmaceuticals. Solvay Pharmaceuticals is helping the world improve its armoury against a possible pandemic with its new cell culture methodology for vaccine production. It is therefore assumed that the development of organs cannot be simulated in cell culture in the foreseeable future and that this must at present be regarded as rather unrealistic. However, in cell culture the most atypic cells express CCHCR1 less than immortalized HaCaT cells, and tumor promotion and induced proliferation of HaCaT cells correlates with reduced CCHCR1 expression. The tests use in-vitro cell culture to evaluate the potential skin-irritancy of chemicals, by providing a realistic representation of the properties of human skin to identify irritant and non-irritant chemicals. We carry out skin and ocular tolerance tests to assure the product's inoffensiveness, on volunteers after individual application for 48 hours or on a cell culture. An auxin-autotrophic maize cell, maize protoplast, maize cell culture or maize callus which is resistant to aryloxyphenoxyalkanecarboxylic acid herbicides. Clamydia trachomatis must be searched for systematically using direct immunofluorescence and by cell culture for the various sites: tubes, ampulla, peritoneum, adhesions. Theratechnologies also has well-equipped discovery and preclinical research laboratories which include two cell culture rooms and several chemical hoods. Despite the fact that synergy was not observed when oxaliplatin was combined with 5-FU in HT-29 cell culture in vitro, oxaliplatin was additively effective with 5-FU against human colonic tumour xenografts in vivo. After 10 min of incubation, transfection mixture was added to cell culture dishes. Material from carcases and cloacal swabs were submitted for virological examination by inoculation into both embryonated fowl eggs and cell culture. Strains will be grown on conventional media and also passaged through in vitro cell culture and embryonated eggs, and then hybridised against the Campychip microarray for comparison. Mycoplasma can be difficult to detect in cell culture because many species do not produce turbidity or cytopathic effects. These with polycarbonate membranes in larger pore sizes are adaptable to many uses in cell culture including transport studies, toxicity tests, chemotaxis studies and electron microscope use. Show More Sentences Picornavirus, rhabdovirus, and HIV in cell culture supernatants retain infectivity after RNAlater stabilization. In preliminary experiments an electroperforation technique was used to transfer the gene for a fluorescent marker protein into myoblasts in cell culture. An in vitro approach using hepatocytes derived from livers of embryonic chickens demonstrated the efficacy of using cell culture as a means to compare the relative potencies of different priority chemicals. A process of harvesting a herpesvirus from a cell culture infected therewith, which comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension. This document complements other guidelines to provide a comprehensive approach to quality issues arising from biological aspects of processing products from metazoan and microbial cell culture. In cell culture models of the BBB, Fas ligand increases T lymphocyte adhesion and permeability, while TRAIL reduces permeability without promoting adhesion. Xyntha is inherently free from the risk of transmission of human blood borne pathogens, because it is manufactured in a cell culture medium that does not contain any materials derived from human or animal sources. Polyvinyl alcohol EMPROVE® is used frequently as an additive in fermentation and cell culture to lower shear forces and thereby improve the processes taking place. Health Canada reviewed scientific evidence from animal, cell culture, and epidemiological studies carried out worldwide and conducted its own studies, which are published in peer-reviewed journals. Previous studies on dental pulp cell culture have described heterogenous mixtures of cells that differentiate into odontoblasts and form mineralized dentin. Influenza surface antigen vaccine from Influenza Viruses propagated on animal cell culture obtainable by the method of claim 3 and having a host cell DNA content equal to or less than 25 pg per dose. Stimulation of growth and mucopolysaccharide synthesis by insulin treatment of chick embryo chondrocytes in cell culture. This was unexpected since the spores would have to germinate on a component of the oil in order to produce a vegetative cell culture. Kingfisher Biotech's mouse GM-CSF protein has been shown to be a superior product for use in cell culture. Plant cell culture For SWCNTs added to the growth media of plant cell cultures, positive as well as negative effects have been observed, depending on the applied dosage. Figure 2 Influence of cell culture conditions. Only viruses which can be propagated and assessed for viability in cell culture can be accepted as we do not have suitable resources to handle viruses which require an animal host or more exotic methodology. Development of a cell culture system susceptible to measle, canine distemper, and rinderpest viruses. The KCLRF laboratories are equipped with modern facilities for cell culture and long-term storage as well as equipment for handling radioactive isotopes. Unfortunately, the impact of cell culture contamination extended far beyond the relatively narrow field of cytobiology and the researchers studying cell lines. Show More Sentences The cell culture media segment has been sub-segmented into chemically defined, classical, lysogeny broth, protein-free, serum-free, and specialty media. There was no evidence of terminal keratinization in the cell cultures, and cells could be serially passaged indefinitely using standard cell culture methods. The disposable bioreactors are designed for use in a shaking incubator, permitting passive mass transfer to the cell culture medium by the shaking mechanism. Find more words! 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Use our Sentence Generator Nearby Words cell body cellblock cellars cellarman cellarette cellaret cell cycle cell differentiation cell division celled celli celling